Description
Human Phosphoserine phosphatase (hPSP) is an important enzyme in the phosphorylated pathway of serine biosynthesis, which contributes a major portion of the endogenous L-serine. Similar to known L-3-phosphoserine phosphatases, it catalyzed the Mg2+-dependent hydrolysis of L-phosphoserine and an exchange reaction between L-serine and L-phosphoserine. Recently, its complex structures reveal that the open-closed environmental change of the active site, generated-helical bundle domain, is important to substrate by local rearrangement of the recognition and hydrolysis.
Abbr
PSP, Recombinant (Human)
Appearance
Sterile filtered colorless solution.
Product Overview
Phosphoserine Phosphatase Human Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 225 amino acids and having a molecular mass of 25 kDa. PSP was overexpressed in E. coli and purified by conventional chromatography.
Enzyme Commission Number
EC 3.1.3.3
Purity
Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Buffer
The protein contains 20mM Hepes pH 7.5, 1mM DTT &100mM KCl2.
Synonyms
Phosphoserine phosphatase; EC 3.1.3.3; PSP; O-phosphoserine phosphohydrolase; PSPase; L-3-phosphoserine phosphatase; PSPH
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