Description
Pectin acetylesterase catalyses the hydrolysis of acetate from acetylated pectins.
Abbr
PAE, Recombinant (Clostridium thermocellum)
Source
Clostridium thermocellum
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.1.1.-
Molecular Weight
23800 Da
Purity
> 95 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of p-nitrophenol per minute from p-nitrophenyl acetate (1 mM in the assay) in 50 mM phosphate buffer, pH 6.5, at 50°C, containing 1 mg/ml of BSA.
Optimum pH
6.5 (stable from 6 – 7.5)
Optimum temperature
50°C (stable up to 60°C)
Storage
Store at 4°C (shipped at room temperature)
Preparation Instructions
Agitate vial sufficiently to fully homogenise enzyme precipitate before use.
Synonyms
Pectinacetylesterase; PAE
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