Applications
o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Enzyme Commission Number
202-430-6
Synonyms
1, 2-Diaminobenzene; 1, 2-Phenylenediamine; OPD
PubChem Substance ID
24899004
Vapor pressure
0.01 mmHg ( 25 °C)
Beilstein Registry Number
606074
Substrates
Peroxidase Substrates
Vapor Density
3.7 (vs air)
Impurities
λex 337 nm; λem 417 nm (Pyruvic acid derivative)
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