Applications
o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Enzyme Commission Number
202-430-6
Synonyms
1, 2-Diaminobenzene; 1, 2-Phenylenediamine; OPD
PubChem Substance ID
24898638
Vapor pressure
0.01 mmHg ( 25 °C)
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
Beilstein Registry Number
606074
Substrates
Peroxidase Substrates
Vapor Density
3.7 (vs air)
Impurities
λex 337 nm; λem 417 nm (after derivatization)
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