Products
Online Inquiry
Our Products Cannot Be Used As Medicines Directly For Personal Use.
Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.
o-Phenylenediamine dihydrochloride
Cat No.
CSUB-0429
Applications
o-Phenylenediamine is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Form
tablet
Enzyme Commission Number
210-418-7
CAS No.
615-28-1
Molecular Weight
181.06
Storage
2-8°C
MDL Number
MFCD00012966
PubChem Substance ID
24898457
mp
258 °C (dec.)(lit.)
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
Beilstein Registry Number
3912045
Substrates
Peroxidase Substrates
Download
Download Datasheet