Description
In enzymology, an oligogalacturonide lyase (EC 4.2.2.6) is an enzyme that catalyzes the chemical reaction: 4-(4-deoxy-beta-D-gluc-4-enuronosyl)-D-galacturonate → 2 5-dehydro-4-deoxy-D-glucuronate. Hence, this enzyme has one substrate, 4-(4-deoxy-beta-D-gluc-4-enuronosyl)-D-galacturonate, and one product, 5-dehydro-4-deoxy-D-glucuronate. This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides.
Abbr
Oligogalacturonate lyase, Recombinant (Dickeya dadantii)
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 4.2.2.6
Molecular Weight
46.2 kDa
Purity
>90% as judged by SDS-PAGE
Specificity
Oligogalacturonides
Optimum temperature
30 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
Oligogalacturonide lyase; unsaturated oligogalacturonate transeliminase; OGTE; EC 4.2.2.6