Enzyme Commission Number
243-737-5
Specificity
o-Dianisidine (3,3'-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.
Synonyms
3, 3'-Dimethoxybenzidine dihydrochloride; Fast Blue B
Molecular Formula
C14H16N2O2 · 2HCl
PubChem Substance ID
24894263
Reconstitution
Dissolve one tablet in 60 ml of 50 mM phosphate-citrate buffer, pH 5.0. Add 12 μl of fresh 30% hydrogen peroxide immediately before to use.
Beilstein Registry Number
3917996
Substrates
Peroxidase Substrates
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