Description
In enzymology, a N-methylhydantoinase (ATP-hydrolysing) is an enzyme that catalyzes the chemical reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O rightleftharpoons ADP + phosphate + N-carbamoylsarcosine. The 3 substrates of this enzyme are ATP, N-methylimidazolidine-2,4-dione, and H2O, whereas its 3 products are ADP, phosphate, and N-carbamoylsarcosine. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amides. This enzyme participates in arginine, creatinine, and proline metabolism.
Abbr
N-Methylhydantoinase, Recombinant (Arthrobacter sp.)
Applications
Use N-Methylhydantoinase (ATP-hydrolyzing) in diagnostic tests for the determination of creatinine in combination with Creatinine Deaminase, N-Carbamoylsarcosine Amidase and Sarcosine Oxidase.
Appearance
White lyophilizate
Product Overview
Hydrolase for creatinine determination that uses ATP to catalyze the conversion of N-methylhydantoin to N-carbomoylsarcosine.
Contaminants
Creatinase: <0.013 Creatininase: <0.01 Catalase: <100 Uricase: <0.01
Stability
At -15 to -25°C within specification range for 12 months. Store dry. Protect from light.
Synonyms
N-methylimidazolidine-2,4-dione amidohydrolase (ATP-hydrolysing); N-methylhydantoin amidohydrolase; methylhydantoin amidase; N-methylhydantoin hydrolase; N-methylhydantoinase
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