Description
Nitrilase enzymes catalyse the hydrolysis of nitriles to carboxylic acids and ammonia, without the formation of "free" amide intermediates. Nitrilases are involved in natural product biosynthesis and post translational modifications in plants, animals, fungi and certain prokaryotes. Nitrilases can also be used as catalysts in preparative organic chemistry. Among others, nitrilases have been used for the resolution of racemic mixtures.
Abbr
Nitrilase, Recombinant
Package
Bottomless glass bottle. Contents are inside inserted fused cone.
Product Overview
The isoelectric point (pI) of this protein is 8.1. Native PAGE analysis indicates that the active complex of this enzyme consists of 14 identical subunits.
Enzyme Commission Number
EC 3.5.5.1
Molecular Weight
mol wt 41 kDa
Unit Definition
1 U corresponds to the amount of enzyme which liberates 1 μmol ammonia per minute at pH 7.2 and 25°C with the conversion of acrylonitrile to acrylic acid
Synonyms
nitrilase; acetonitrilase; benzonitrilase; EC 3.5.5.1; 9024-90-2
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