Description
Requires Fe(III). The enzyme from the bacterium Streptoalloteichus tenebrarius catalyses the activation of carbamoyl phosphate to O-carbamoyladenylate and the subsequent carbamoylation of kanamycin and tobramycin.
Form
Liquid or lyophilized powder
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
Synonyms
tobramycin carbamoyltransferase; TobZ
Reaction
(1) tobramycin + carbamoyl phosphate + ATP + H2O = nebramycin 5' + AMP + diphosphate + phosphate (overall reaction); (1a) carbamoyl phosphate + ATP + H2O = diphosphate + O-carbamoyladenylate + phosphate; (1b) O-carbamoyladenylate + tobramycin = AMP + nebramycin 5'; (2) kanamycin A + carbamoyl phosphate + ATP + H2O = 6''-O-carbamoylkanamycin A + AMP + diphosphate + phosphate (overall reaction); (2a) carbamoyl phosphate + ATP + H2O = diphosphate + O-carbamoyladenylate + phosphate; (2b) O-carbamoyladenylate + kanamycin A = AMP + 6''-O-carbamoylkanamycin A
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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