Description
Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closed-circular DNA. Enzyme activity is independent of right-and left-handed superhelices.
Abbr
TOPO I, Native (Wheat germ)
Applications
Suitable for: • Analsis of degree of DNA supercoiling • Forming knots and circles in single stranded DNA • Converting complementary single stranded DNA to double stranded, circular forms • Rejoining nicks in double-stranded DNA
Product Overview
Topoisomerase I relaxes supercoiled DNA molecules. The enzyme initiates transient breakages and rejoins of phosphodiester bonds in superhelical turns of closedcircular dsDNA.
Form
buffered aqueous glycerol solution
Enzyme Commission Number
EC 5.99.1.2
Molecular Weight
mol wt 97 kDa
Unit Definition
One unit will convert 1μg of supercoiled pGEM9Zf (−) plasmid DNA to the relaxed form in 30 min at 37°C.
Warnings
To preserve enzyme activity, avoid multiple freeze-thaw cycles and frequent temperature variations.
Synonyms
Topoisomerase I; EC 5.99.1.2; type I DNA topoisomerase; untwisting enzyme; relaxing enzyme; nicking-closing enzyme; swivelase; ω-protein; deoxyribonucleate topoisomerase; topoisomerase; type I DNA topoisomerase; DNA topoisomerase; TOPO I
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