Description
Transketolase is highly specific for ketol donor substrates and is stereospecific and enantioselective to hydroxyaldehyde substrates with an (R) configuration. It specifically catalyzes the irreversible transfer of one ketol unit from α-hydroxypyruvic acid to an aldehyde to produce a D-threo (3S,4R)ketose.
Abbr
Transketolase, Native (Thermophillic bacteria)
Source
Thermophillic bacteria
Applications
This enzyme is a potential candidate for biocatalysis, suitable for pharmaceutical development / manufacturing. Asymmetric C-C bond formation, ketol donor D-xylulose-5-phosphate may be substituted by hydroxypyruvate; preparation of ketose sugars such as fructose analogs, azasugars and fluorogenic substrates.
Enzyme Commission Number
EC 2.2.1.1
Unit Definition
One unit is defined as the amount of enzyme producing 1 µmol of D-glyceraldehyde 3-phosphate using ribose-5-phosphate and xylulose-5-phosphate from ribulose-5-phosphate with ribulose-phosphate-3-epimerase per minute.
Buffer
20mM Tris-HCl(pH 8.0), 1 mM DTT, 5 mM MgCl2, 10 mM NaCl
Synonyms
Transketolase; EC 2.2.1.1; 9014-48-6; glycolaldehydetransferase; Glycolaldehyde Transferase
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