Products
Online Inquiry
Our Products Cannot Be Used As Medicines Directly For Personal Use.
Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.
Native Thermoactinomyces intermedius Phenylalanine Dehydrogenase
Cat No.
NATE-1906
Description
Phenylalanine dehydrogenase is a member of a large family of amino-acid dehydrogenases, which includes glutamate dehydrogenase, alanine dehydrogenase, leucine dehydrogenase, lysine €-dehydrogenase, and meso-a,€-diaminopimelate D-dehydrogenase. The three known gene sequences are octomers. It has a two-domain, three-dimensional structure.
Abbr
PheDH, Native (Thermoactinomyces intermedius)
Alias
PheDH
Source
Thermoactinomyces intermedius
Appearance
Ammonium sulphate suspension
Enzyme Commission Number
EC 1.4.1.20
CAS No.
69403-12-9
Contaminants
(as PheDH activity = 100 %)
NADH oxidase: < 0.01 %;
Lactate dehydrogenase: < 0.01 %.
NADH oxidase: < 0.01 %;
Lactate dehydrogenase: < 0.01 %.
Molecular Weight
ca. 380,000; Subunit molecular weight : ca. 40,000.
pH Stability
5.0 - 10.0
Michaelis Constant
(200 mM Gly-KCl-KOH buffer, pH 11.0, at 30 °C)
L-Phenylalanine: 0.66 mM;
NAD+: 0.05 mM.
L-Phenylalanine: 0.66 mM;
NAD+: 0.05 mM.
Specificity
L-Phenylalanine: 100 %;
L-Tyrosine: 7.6 %;
L-Methionine: 1.5 %.
L-Tyrosine: 7.6 %;
L-Methionine: 1.5 %.
Unit Definition
One unit of activity is defined as the amount of PheDH that forms 1 μmol of NADH per minute at 30 °C.
Optimum pH
11.5
Thermal stability
No detectable decrease in activity up to 50 °C.
Storage
Stable at 0 to 4 °C for at least six months (Do not freeze).
Synonyms
phenylalanine dehydrogenase; EC 1.4.1.20; L-phenylalanine dehydrogenase; PHD; 69403-12-9
Specific Activity
more than 30 U/mg protein
Reaction
L-Phenylalanine + NAD+ + H2O ←→ Phenylpyruvate + NH4+ + NADH
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1803 | L-Phenylalanine Dehydrogenase (Crude Enzyme) | EC 1.4.1.20 | 69403-12-9 | E. coli | Inquiry |
| NATE-0558 | Native Sporosarcina sp. L-Phenylalanine Dehydrogenase | EC 1.4.1.20 | 69403-12-9 | Sporosarcina sp. | Inquiry |
| EXWM-1454 | phenylalanine dehydrogenase | EC 1.4.1.20 | 69403-12-9 | Inquiry |