Description
Polynucleotide kinase catalyses a "forward reaction" transfer of the γ-phosphate of ATP to the 5' hydroxyl terminus of single-and double-stranded nucleic acids (DNA and RNA) and 3'-nucleoside monophosphates. In exchange reactions containing ADP, the enzyme will catalyze the exchange of 5'-terminal phosphate groups and ATP. The 3'-phosphatase activity enables the enzyme to remove 3'-phosphoryl groups from phosphorylpolynucleotides.
Abbr
PNK, Native (T4-infected Escherichia coli)
Source
T4-infected Escherichia coli
Applications
Suitable for: • Sequencing or nucleic acid tagging (DNA and RNA) by 5′-end labeling • 5′ phosphorylation of oligonucleotides • Removal of 3′-phosphate groups from phosphorylpolynucleotides
Form
buffered aqueous glycerol solution
Enzyme Commission Number
EC 2.7.1.78
Molecular Weight
mol wt 33 kDa
Concentration
10 units/μL
Unit Definition
One unit catalyzes the transfer of one nanomole of 32P to the 5'-end of micrococcal nuclease-treated DNA in 30 min. at 37°C. Transfer is detected as incorporation into acid-insoluble material.
Warnings
Activity is determined in a reaction mixture containing 40 mM Tris-HCl (pH 7.5), with 10 mM MgCl2, 5 mM dithiothreitol, 0.5 mM 5’-OH polynucleotide ends, and mM [γ-32P]-ATP.
Synonyms
polynucleotide 5'-hydroxyl-kinase; EC 2.7.1.78; 37211-65-7; ATP:5'-dephosphopolynucleotide 5'-phosphatase; PNK; polynucleotide 5'-hydroxyl kinase (phosphorylating); 5'-hydroxyl polynucleotide kinase; 5'-hydroxyl polyribonucleotide kinase; 5'-hydroxyl RNA kinase; DNA 5'-hydroxyl kinase; DNA kinase; polynucleotide kinase; polynucleotide 5'-hydroxy-kinase
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