Description
β-glucanases degrade β-1,4-glucans of cellulose, xyloglucan and β-1,4-xylan. β-Glucanase represents a group of carbohydrate enzymes which break down glycosidic bonds within beta-glucan. It forms the main constituent of fungal cell walls and could be a potential structural and storage polysaccharide of marine macro-algae. It has the ability to degrade fungal cell walls and may be involved in defense mechanism of plants against pathogenic fungi.
Source
Rhodothermus marinus
Enzyme Commission Number
EC 3.2.1.6
Structure
The crystal structure of Laminarinase from Rhodothermus marinus (96% sequence identity with Bglu110 Laminarinase/Lichenase) has been determined to 1.9 Å resolution. – PDB entry 3ILN
Specificity
The molecule laminarin (also known as laminaran) is a storage glucan produced in brown algae through photosynthesis. The polysaccharide is made up of glucose residues withβ-1,3-linkages and β-1,6-linkages. It is a linear polysaccharide, with a β (1→3):β (1→6) ratio typically 3:1 but the ratio may vary with the source of the polysaccharide. Lichenin, also known as lichenan or moss starch, is a complex glucan occurring in certain species of lichensandconsists of repeating glucose units linked by β-1,3 and β-1,4 glycosidic bonds. It can be extracted from Cetraria islandica (Iceland moss). Scleroglucan, is formed by the fungus Sclerotium rolfsii. A chemically analogous polysaccharide, Schizophyllan (Sizofiran, Sonifilan, SPG) is a neutral extracellular polysaccharide produced by the fungus Schizophyllum commune. Schizophyllan is a β-1,3 beta-glucan with β-1,6 branching. Both polysaccharides share the chemical structure of the backbone with curdlan.
Unit Definition
One unit (U) of enzyme activity is the amount that leads to the release of 1 μmol reducing sugars per minute.
Optimum pH
the enzyme has optimum activity around pH 7
Optimum temperature
the enzyme has optimum activity around 80°C
Thermal stability
The ß-galactosidase heat stability at 70,80 and 91°C
Synonyms
endo-1,3-β-D-glucanase; laminarinase; laminaranase; β-1,3-glucanase; β-1,3-1,4-glucanase; endo-1,3-β-glucanase; endo-β-1,3 (4)-glucanase; endo-β-1,3-1,4-glucanase; endo-β-(1→3)-D-glucanase; endo-1,3-1,4-β-D-glucanase; endo-β-(1-3)-D-glucanase; endo-β-1,3-glucanase IV; endo-1,3-β-D-glucanase; 1,3-(1,3; 1,4)-β-D-glucan 3 (4)-glucanohydrolase; EC 3.2.1.6; 9074-98-0
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