Description
Sphingolipid ceramide N-deacylase (SCDase) is derived from Pseudomonas and hydrolyzes the N-acyl linkage between fatty acids and sphingosine bases in ceramides of various sphingolipids. The enzyme also catalyzes the reverse reaction and possesses transacylation activity. SCDase acts on various acidic and neutral glycosphingolipids and sphingomyelin; however, it exhibits low activity with ceramides.
Abbr
SCDase, Native (Pseudomonas sp.)
Applications
Hydrolysis of N-acyl linkages between fatty acids and sphingosine bases Sphingolipid hydrolysis
Form
Solution in 50 mM sodium acetate (pH 6.0) containing 0.1% Lubrol PX
Unit Definition
One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1 µmol of asialo GM1 per minute.
Storage
Store at –20°C until use. Store reconstituted solution in aliquots at –20°C. Avoid freeze-thaw.
Inhibitors
Hg2+, Zn2+ and Cu2+
Synonyms
SCDase; Sphingolipid ceramide N-deacylase
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