Description
In enzymology, sarcosine dehydrogenase (EC 1.5.99.1) is a mitochondrial enzyme that catalyzes the chemical reaction N-demethylation of sarcosine to give glycine. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donor with other acceptors. Sarcosine dehydrogenase is closely related to dimethylglycine dehydrogenase, which catalyzes the demethylation reaction of dimethylglycine to sarcosine. Both sarcosine dehydrogenase and dimethylglycine dehydrogenase use FAD as a cofactor. Sarcosine dehydrogenase is linked by electron-transferring flavoprotein (ETF) to the respiratory redox chain.
Abbr
Sarcosine dehydrogenase, Native (Pseudomonas sp.)
Form
Lyophilized powder containing approx. 60% sucrose, 10% potassium phosphate buffer salts and trace EDTA
Enzyme Commission Number
EC 1.5.99.1
Activity
0.5-1.5 units/mg protein
Unit Definition
One unit will convert 1.0 μmole of sarcosine to glycine and formaldehyde per min at pH 7.5 at 37°C.
Synonyms
sarcosine dehydrogenase; EC 1.5.99.1; sarcosine N-demethylase; monomethylglycine dehydrogenase; sarcosine: (acceptor) oxidoreductase (demethylating); 37228-65-2; EC 1.5.8.3
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