Description
The Long chain fatty acyl-CoA synthetase enzyme is a member of the ligase family that activates the breakdown of complex fatty acids. Long chain fatty acyl-CoA synthetase plays a crucial role in intermediary metabolism by catalyzing the formation of fatty acyl-CoA by a two-step process proceeding through an adenylated intermediate. It is an enzyme present in all organisms from bacteria to humans. It catalyzes the pre-step reaction for β-oxidation of fatty acids or can be incorporated in phospholipids.
Abbr
ACS, Native (Pseudomonas fragi)
Applications
Useful in the enzymatic determination of fatty acid when coupled with Acyl-CoA oxidase
Product Overview
ACS is an enzyme produced by microorganisms. This product shall be used for a diagnostics reagent.
Enzyme Commission Number
EC 6.2.1.3
Contaminants
Catalase < 1.00%; Glucose oxidase < 0.1%
Molecular Weight
60 kDa (Sephadex G‒150) SDS‒PAGE 62 kDa
pH Stability
6.0-8.0 (37°C, 2 hrs)
Michaelis Constant
Palmitic acid 1.1 × 10-5M; ATP 1.7 × 10-4M; CoA 3.2 × 10-4M
Unit Definition
One unit is defined as the amount of enzyme which converts 1 μmole of fatty acid to acyl‒CoA per minute at 37°C under the conditions specified in the assay procedure.
Thermal stability
Stable at 50°C and below (pH 7.5, 10 mins)
Stability
At least one year at-20°C
Storage
Keep in freezer (-20°C to-80°C), dry place in well-closed containers and away from direct sun light).
Synonyms
EC 6.2.1.3; ACS; acyl-CoA synthetase; fatty acid thiokinase (long chain); acyl-activating enzyme; palmitoyl-CoA synthase; lignoceroyl-CoA synthase; arachidonyl-CoA synthetase; acyl coenzyme A synthetase; acyl-CoA ligase; palmitoyl coenzyme A synthetase; thiokinase; palmitoyl-CoA ligase; acyl-coenzyme A ligase; fatty acid CoA ligase; long-chain fatty acyl coenzyme A synthetase; oleoyl-CoA synthetase; stearoyl-CoA synthetase; long chain fatty acyl-CoA synthetase; long-chain acyl CoA synthetase; fatty acid elongase; LCFA synthetase; pristanoyl-CoA synthetase; ACS3; long-chain acyl-CoA synthetase I; long-chain acyl-CoA synthetase II; fatty acyl-coenzyme A synthetase; long-chain acyl-coenzyme A synthetase; FAA1
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