Description
In enzymology, an aldose 1-epimerase (EC 5.1.3.3) is an enzyme that catalyzes the chemical reaction:alpha-D-glucose↔ beta-D-glucose. Hence, this enzyme has one substrate, alpha-D-glucose, and one product, beta-D-glucose. This enzyme belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and derivatives. This enzyme participates in glycolysis and gluconeogenesis.
Abbr
Mutarotase, Native (Porcine)
Applications
Clinical Chemistry
Form
A suspension in 80% saturated ammonium sulphate solution, pH 8.0
Enzyme Commission Number
EC 5.1.3.3
Unit Definition
The amount of enzyme causing an increase in the rate of spontaneous mutarotation of D-glucose of one micromole per minute at 25°C and pH 7.4.
One unit as described above is equivelent to approximately 0.04 units in a glucose dehydrogenase coupled assay at 25°C and pH
7.2 or approximately 0.0035 units in a GO/HRP coupled assay at 37°C and pH 7.2.
Synonyms
mutarotase; aldose mutarotase; galactose mutarotase; galactose 1-epimerase; D-galactose 1-epimerase; aldose 1-epimerase; EC 5.1.3.3
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