Description
In enzymology, an aldose 1-epimerase (EC 5.1.3.3) is an enzyme that catalyzes the chemical reaction:alpha-D-glucose↔ beta-D-glucose. Hence, this enzyme has one substrate, alpha-D-glucose, and one product, beta-D-glucose. This enzyme belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and derivatives. This enzyme participates in glycolysis and gluconeogenesis.
Abbr
Mutarotase, Native (Microorganism)
Applications
This enzyme is useful for enzymatic determination of glucose.
Enzyme Commission Number
EC 5.1.3.3
Contaminants
(as MRO activity = 100 %)
NADHoxidase: < 0.01 %
Molecular Weight
ca. 39,500
Unit Definition
One unit of activity is defined as the amount of Mutarotase that forms 10μmol of NADH per minute at 25 °C.
Thermal stability
No detectable decrease in activity up to 50 °C.
Storage
Stable at -20 °C for at least one year.
Synonyms
mutarotase; aldose mutarotase; galactose mutarotase; galactose 1-epimerase; D-galactose 1-epimerase; aldose 1-epimerase; EC 5.1.3.3
Specific Activity
more than 120 U/mg protein
Reaction
α-D-glucose ←→ β-D-glucose
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