Description
Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.
Abbr
Fumarase, Native (Porcine)
Applications
Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.
Product Overview
Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mit ochondria of all eukaryotes.
Form
ammonium sulfate suspension; Suspension in 3.2 M (NH4)2SO4, 0.05 M KH2PO4, pH 7.5, 0.014 M 2-mercaptoethanol
Enzyme Commission Number
EC 4.2.1.2
Activity
300-500 units/mg protein (biuret)
Unit Definition
One unit will convert 1.0 μmole of L-malate to fumarate per min at pH 7.6 at 25°C.
Warnings
Isolated by the method of Beeckmans, S., and Kanarek, L., Eur. J. Biechem., 78, 437 (1977). Affinity purified.
Synonyms
EC 4.2.1.2; fumarase; L-malate hydro-lyase; (S)-malate hydro-lyase; 9032-88-6
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