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Native Microorganism Sorbitol Dehydrogenase
Cat No.
NATE-1909
Description
Sorbitol dehydrogenase (or SDH) is a cytosolic enzyme. In humans this protein is encoded by the SORD gene. Sorbitol dehydrogenase is an enzyme in carbohydrate metabolism converting sorbitol, the sugar alcohol form of glucose, into fructose. Together with aldose reductase, it provides a way for the body to produce fructose from glucose without using ATP. Sorbitol dehydrogenase uses NAD+ as a cofactor; its reaction is sorbitol + NAD+--> fructose + NADH + H+. A zinc ion is also involved in catalysis. Organs that use it most frequently include the liver and seminal vesicle; it is found in all kinds of organisms from bacteria to humans. A secondary use is the metabolism of dietary sorbitol, though sorbitol is known not to be absorbed as well in the intestine as its related compounds glucose and fructose, and is usually found in quite small amounts in the diet (except when used as an artificial sweetener).
Abbr
SorDH, Native (Microorganism)
Alias
SorDH
Source
Microorganism
Applications
This enzyme is useful for determination of D-Sorbitol in clinical analysis and food analysis.
Appearance
Lyophilized
Enzyme Commission Number
EC 1.1.1.14
CAS No.
9028-21-1
Contaminants
(as SorDH activity = 100 %)
NADH oxidase: <0.01 %
NADH oxidase: <0.01 %
Molecular Weight
ca. 68,000; Subunit molecular weight : ca. 26,000.
pH Stability
6.0 - 10.0
Michaelis Constant
(100 mM Tris-HCl buffer, pH 9.0, at 30°C)
D-Sorbitol: 3.4 mM;
NAD+: 0.13 mM.
D-Sorbitol: 3.4 mM;
NAD+: 0.13 mM.
Specificity
D-Sorbitol: 100 %;
Galactitol: 27 %;
L-Iditol: 42 %;
Xylitol: 1 %;
D-Arabitol: 0 %;
D-Mannitol: 0 %;
D-Glucose: 0 %;
D-Galactose: 0 %;
Maltose: 0 %.
Galactitol: 27 %;
L-Iditol: 42 %;
Xylitol: 1 %;
D-Arabitol: 0 %;
D-Mannitol: 0 %;
D-Glucose: 0 %;
D-Galactose: 0 %;
Maltose: 0 %.
Unit Definition
One unit of activity is defined as the amount of SorDH that forms 1 μmol of NADH per minute at 30 °C.
Optimum pH
11
Optimum temperature
40 °C
Thermal stability
No detectable decrease in activity up to 35 °C.
Storage
Stable at -20 °C for at least one year.
Synonyms
Sorbitol Dehydrogenase; SDH; EC 1.1.1.14; 9028-21-1; L-iditol 2-dehydrogenase; polyol dehydrogenase; sorbitol dehydrogenase; L-iditol:NAD+ 5-oxidoreductase; L-iditol (sorbitol) dehydrogenase; glucitol dehydrogenase; L-iditol:NAD+ oxidoreductase; NAD+-dependent sorbitol dehydrogenase; NAD+-sorbitol dehydrogenase
Specific Activity
more than 30 U/mg protein
Reaction
D-Sorbitol + NAD+ ←→ D-Fructose + NADH + H+
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-0667 | Native Rat Sorbitol Dehydrogenase | EC 1.1.1.14 | 9028-21-1 | Rat Liver | Inquiry |
| NATE-0668 | Native Sheep Sorbitol Dehydrogenase | EC 1.1.1.14 | 9028-21-1 | Sheep liver | Inquiry |
| EXWM-0043 | L-iditol 2-dehydrogenase | EC 1.1.1.14 | 9028-21-1 | Inquiry |