Description
Glucose-6-phosphate dehydrogenase (G6PD or G6PDH) (EC 1.1.1.49) is a cytosolic enzyme that catalyzes the chemical reaction:D-glucose 6-phosphate + NADP+ ↔ 6-phospho-D-glucono-1,5-lactone + NADPH + H+. This enzyme is in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH).
Abbr
G6PDH (Microorganism)
Alias
G6PDH; GPD; G6PD1; G6PD
Applications
The enzyme is useful for enzymatic determination of NAD+(NADP+) and G-6-P, and activities of phosphoglucose isomerase, phosphoglucomutase and hexokinase. The enzyme is also used for enzymatic determination of glucose and creatine phosphokinase activity when coupled with hexokinase.
Appearance
White amorphous powder, lyophilized
Enzyme Commission Number
EC 1.1.1.49
Activity
GradeⅢ 200U/mg-solid or more
Contaminants
Creatine phosphokinase < 1×10⁻³% Phosphoglucomutase < 1×10⁻³% 6-Phosphogluconate dehydrogenase < 5×10⁻³% Phosphoglucose isomerase < 1×10⁻²% Glutathione reductase < 1×10⁻³% Hexokinase < 1×10⁻²% Myokinase < 1×10⁻²% NADH oxidase < 1×10⁻²% NADPH oxidase < 1×10⁻²%
Molecular Weight
approx. 140 kDa (by gel filtration)
pH Stability
pH 5.0-11.0 (25°C, 22hr)
Michaelis Constant
NAD⁺ linked 2.4×10⁻⁴M (NAD⁺), 4.7×10⁻⁴M (G-6-P), NADP⁺ linked 7.4×10⁻⁶M (NADP⁺), 3.2×10⁻⁴M (G-6-P)
Optimum temperature
50°C-55°C
Thermal stability
below 50°C (pH 7.8, 30min)
Stability
Stable at-20°C for at least one year
Inhibitors
Metal ions, iodoacetamimide, SDS etc.
Synonyms
Glucose-6-phosphate dehydrogenase; G6PD; G6PDH; Glucose-6-phosphate dehydrogenase (NADP(+)); EC 1.1.1.49; Glucose-6-phosphate 1-dehydrogenase; Glucose-6-phosphate dehydrogenase; GPD
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