Description
In enzymology, a creatinine deaminase (EC 3.5.4.21) is an enzyme that catalyzes the chemical reaction: creatinine + H2O ↔ N-methylhydantoin + NH3. Thus, the two substrates of this enzyme are creatinine and H2O, whereas its two products are N-methylhydantoin and NH3. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amidines. The systematic name of this enzyme class is creatinine iminohydrolase.
Abbr
Creatinine Deiminase (Microorganism)
Applications
This enzyme is useful for enzymatic determination of creatinine when coupled with glutamate dehydrogenase in clinical analysis.
Appearance
White amorphous powder, lyophilized
Enzyme Commission Number
EC 3.5.4.21
Activity
GradeⅢ 10U/mg-solid or more (containing approx. 30% of stabilizer)
Contaminants
Creatinine amidohydrolase < 1.0×10⁻²% Creatine amidinohydrolase < 1.0×10⁻²% Urease < 1.0×10⁻²% NADH oxidase < 1.0×10⁻²% NH4⁺ < 1.0×10⁻²% µg/u
Molecular Weight
approx. 260 kDa
pH Stability
pH 7.0-11.0 (30°C, 20hr)
Michaelis Constant
3.5×10⁻³M (Creatinine)
Structure
6 subunits per mol of enzyme
Optimum temperature
65-75°C
Thermal stability
below 65°C (pH 7.5, 1hr)
Stability
Stable at-20°C for at least one year
Inhibitors
Ag⁺, Hg⁺⁺, o-phenanthroline,monoiodoacetate
Synonyms
Creatinine hydrolase; Creatinine deaminase; EC 3.5.4.21
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