Description
Phosphoglucomutase (EC 5.4.2.2) is an enzyme that transfers a phosphate group on an α-D-glucose monomer from the 1' to the 6' position in the forward direction or the 6' to the 1' position in the reverse direction. More precisely, it facilitates the interconversion of glucose 1-phosphate and glucose 6-phosphate.
Abbr
α-phosphoglucomutase (Microbial)
Product Overview
High purity recombinant α-Phosphoglucomutase (Microbial) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Enzyme Commission Number
EC 5.4.2.2
Molecular Weight
MW ~ 59.2 kDa
Isoelectric point
pI ~ 5.7
pH Stability
6.0-9.0 (> 75% control activity after 24 hours at 4°C)
Composition
Amino acid composition has been reported by Squire et al. (1964).
Thermal stability
up to 40°C (> 90% control activity after 15 min.)
Warnings
The enzyme is supplied as a solution containing 50% glycerol plus 0.02% (w/v) sodium azide and should be stored at 4°C. For assay, this enzyme should be diluted in glycylglycine buffer (100 mM), pH 7.4 containing 0.5 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use.
Synonyms
α-phosphoglucomutase; Phosphoglucomutase; EC 5.4.2.2; Phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent); Glucose phosphomutase; Phosphoglucose mutase
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