Description
In enzymology, a mannitol 2-dehydrogenase (EC 1.1.1.67) is an enzyme that catalyzes the chemical reaction:D-mannitol + NAD+↔ D-fructose + NADH + H+. Thus, the two substrates of this enzyme are D-mannitol and NAD+, whereas its 3 products are D-fructose, NADH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. This enzyme participates in fructose and mannose metabolism.
Abbr
Mannitol Dehydrogenase, Native (Leuconostoc mesenteroides)
Source
Leuconostoc mesenteroides
Applications
This preparation is useful in the determination of mannitol in urine. Can be used for the production of D-mannitol. It has been used in a study to assess glucosylglycerol and glucosylglycerate as enzyme stabilizers.
Form
Lyophilized powder containing buffer salts, potassium phosphate, and dithiothreitol
Enzyme Commission Number
EC 1.1.1.67
Activity
> 60 units/mg protein
Unit Definition
One unit will reduce 1.0 μmole of D-fructose per min in the presence of NADH at pH 5.3 at 30°C. One unit of enzyme, as defined above, is equivalent to approximately 1.8 units of enzyme, as described by Lunn et al., one unit will oxidize 1.0 μmole of D-mannitol per min in the presence of NAD at pH 8.6 at 40°C.
Warnings
Partially purified. Assayed according to the method of Yamanaka, Meth. Enzymol., 41 (B), 138 (1975).
Synonyms
mannitol dehydrogenase; D-mannitol dehydrogenase; mannitol dehydrogenase; mannitol 2-dehydrogenase; EC 1.1.1.67; 9001-65-4
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