Description
Glutathione S-transferases are a family of proteins that catalyze the conjugation of reduced glutathione with a variety of hydrophobic chemicals containing electrophilic centers.
Form
Lyophilized powder containing Tris buffer salts, reduced glutathione and EDTA.
Enzyme Commission Number
EC 2.5.1.18
Activity
25-125 units/mg protein
Unit Definition
One unit will conjugate 1.0 μmole of 1-chloro-2,4-dinitrobenzene with reduced glutathione per min at pH 6.5 at 25°C.
Warnings
Protein determined by biuret. Purified and assayed by a modification of the method of Simons and Vander Jagt. Enzymatic activities are based on the conjugation of reduced glutathione with a second substrate. The individual proteins generally have activity with more than one class of substrate.
Pathway
Biological oxidations, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; Drug metabolism-cytochrome P450, organism-specific biosystem; Drug metabolism-cytochrome P450, conserved biosystem; Glutathione conjugation, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem; Arachidonate Epoxygenase / Epoxide Hydrolase, organism-specific biosystem; Biological oxidations, organism-specific biosystem; Diurnally regulated genes with circadian orthologs, organism-specific biosystem; Drug metabolism-cytochrome P450, organism-specific biosystem; Drug metabolism-cytochrome P450, conserved biosystem; Glutathione conjugation, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem
Function
glutathione transferase activity; transferase activity; JUN kinase binding; S-nitrosoglutathione binding; dinitrosyl-iron complex binding; drug binding; glutathione binding; glutathione transferase activity; glutathione transferase activity; kinase regulator activity; nitric oxide binding; protein binding; transferase activity
Synonyms
EC 2.5.1.18; glutathione S-transferase; glutathione S-alkyltransferase; glutathione S-aryltransferase; S-(hydroxyalkyl)glutathione lyase; glutathione S-aralkyltransferase; glutathione S-alkyl transferase; GST; 50812-37-8
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