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Native Hexokinase (ADP-Dependent) from Pyrococcus furiosus

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Cat No.

NATE-1135

Description

In enzymology, a ADP-Dependent Hexokinase (EC 2.7.1.147) is an enzyme that catalyzes the chemical reaction: D‒Glucose + ADP → D‒Glucose‒6‒phosphate + AMP.

Abbr

Hexokinase (ADP-Dependent), Native (Pyrococcus furiosus)

Source

Pyrococcus furiosus

Applications

Useful for the enzymatic determination of ADP.

Appearance

White amorphous powder, lyophilized

Enzyme Commission Number

EC 2.7.1.147

Activity

More than 30 U/mg solid

CAS No.

173585-07-4

Contaminants

NADPH oxidase < 0.01%; ATPase < 0.01%

Molecular Weight

100 kDa (gel filtration) 51 kDa (SDS‒PAGE)

Isoelectric point

5.1

pH Stability

5.0‒10.0

Michaelis Constant

Glucose 0.64 mM (at 37°C) ADP 0.07 mM (at 37°C)

Unit Definition

One unit is defined as the amount of enzyme which converts 1 μmole of glucose to D‒Glucose‒6‒phosphate per minute at 37°C under the conditions specified in the assay procedure.

Optimum pH

7.5

Optimum temperature

100°C

Activators

Mg2+, Co2+, Mn2+

Thermal stability

Stable at 95°C and below

Storage

Storage at －20°C in the presence of a desiccant is recommended.

Synonyms

ADP-dependent glucokinase; ADP-specific glucokinase; ADP:D-glucose 6-phosphotransferase; EC 2.7.1.147

"Hexokinase (ADP-Dependent)" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1136	Native Hexokinase (ADP-Dependent) from Thermococcus litoralis	EC 2.7.1.147	173585-07-4	Thermococcus litoralis	Inquiry
EXWM-2977	ADP-specific glucokinase	EC 2.7.1.147	173585-07-4		Inquiry