Description
Prolyl endopeptidase (PE) also known as prolyl oligopeptidase or post-proline cleaving enzyme is an enzyme that in humans is encoded by the PREP gene.
Abbr
PE (Flavobacterium sp.)
Source
Flavobacterium sp.
Applications
This enzyme is useful for the determination of amino acid sequences of peptides and proteins containing proline residues.
Appearance
White amorphous powder, lyophilized
Enzyme Commission Number
EC 3.4.21.26
Activity
GradeⅠ 5.0U/mg-solid or more
Contaminants
Leucine aminopeptidase < 1.0×10⁻¹% Trypsin-like activity < 1.0×10⁻¹%
Molecular Weight
approx. 78 kDa
pH Stability
5.5-8.5 (30°C,15hr)
Michaelis Constant
2.5×10⁻⁵M (Z-Gly-Pro-MCA), 1.4×10⁻⁴M (Z-Gly-Pro-2NNap)
Specificity
Y-Pro(Ala)-X (Y, peptide or N-protected amino acid; X, amino acid, peptide, amide, or ester)
Optimum temperature
37°C (40°C)
Thermal stability
below 40°C (pH 7.0,10 min)
Inhibitors
DFP, 3, 4-dichloroisocoumarin, Z-Gly-Pro-CH₂Cl
Synonyms
EC 3.4.21.26; post-proline cleaving enzyme; proline-specific endopeptidase; post-proline endopeptidase; proline endopeptidase; endoprolylpeptidase; prolyl endopeptidase; prolyl oligopeptidase; PE
Download Datasheet