Description
Hydrolyses the terminal 2-O-sulphate on the unsaturated di-and oligo-saccharides produced by the action of lyases on sulphated glycosaminoglycans.
Abbr
2-O-Sulfatase, Native (Flavobacterium heparinum)
Source
Flavobacterium heparinum
Applications
2-O-Sulfatase from Flavobacterium heparinum has been used in a study to determine that oversulfated chondroitin sulfate is a contaminant in heparin associated with adverse clinical events. 2-O-Sulfatase from Flavobacterium heparinum has also been used in a study to determine the structure of oligosaccharides prepared from acharan sulfate.
Product Overview
Hydrolyses the terminal 2-O-sulphate on the unsaturated di-and oligo-saccharides produced by the action of lyases on sulphated glycosaminoglycans.
Form
Solution contains 10 mM tris-acetate, pH 7.3, 80 mM NaCl, 10 mM potassium phosphate, 0.2% BSA.
Activity
10-40 (units/ml)
Unit Definition
One unit will hydrolyze heparin disaccharide I-P to yield 1.0 micromole of disaccharide II-P (δ-UA-GlcNCOEt-6S) per minute at pH 7.3 at 25°C.
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