Description
E. coli RNA Polymerase, Holoenzyme is the core enzyme saturated with sigma factor 70. The Holoenzyme initiates RNA synthesis from sigma 70 specific bacterial and phage promoters. E. coli RNA Polymerase, Core Enzyme consists of 5 subunits designated α, α, β´, β, and ω. The enzyme is free of sigma factor and does not recognize any specific bacterial or phage DNA promoters. The enzyme retains the ability to transcribe RNA from nonspecific initiation sequences. Addition of sigma factors will allow the enzyme to initiate RNA synthesis from specific bacterial and phage promoters.
Abbr
RNAP, Native (E. coli)
Applications
RNA synthesis from E. coli promoter Transcription initiation studies In vitro translation with PURExpress
Form
20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol (DTT) and 50% glycerol
Molecular Weight
400 kDa.
Concentration
1,000 units/ml
Unit Definition
One unit is defined as the amount of enzyme required to incorporate 1 nmol NTP into RNA in 10 minutes at 37°C.
Synonyms
E. coli RNA Polymerase, Holoenzyme; E. coli RNA Polymerase; RNA Polymerase; RNAP; RNApol; DNA-dependent RNA polymerase
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