Description
Galactose oxidase is an extracellular copper-containing enzyme, secreted by the deuteromycete fungus Dactylium dendroides. It catalyzes the oxidation of a range of primary alcohols, including D-galactose, to the corresponding aldehyde, with reduction of oxygen to hydrogen peroxide.
Abbr
Galactose Oxidase, Native (Dactylium dendroides)
Source
Dactylium dendroides
Applications
Galactose oxidase may be used as an analytical tool for the specific determination of D-galactose in blood plasma, plant extracts, and phospholipids. It could be used for the characterization of terminal D-galactoside units in several polymers. It may also be useful in the determination of lactose.
Product Overview
Galactose oxidase is an extracellular copper-containing enzyme, secreted by the deuteromycete fungus Dactylium dendroides. It catalyzes the oxidation of a range of primary alcohols, including D-galactose, to the corresponding aldehyde, with reduction of oxygen to hydrogen peroxide.
Form
Type I, Lyophilized, contains buffer salts and stabilizer; Type II, lyophilized powder.
Enzyme Commission Number
EC 1.1.3.9
Activity
Type I, 500-1,500 units/mg protein; Type II, > 3000 units/g solid.
Unit Definition
One unit will produce a ΔA425 of 1.0 per min at pH 6.0 at 25°C, in a peroxidase and o-tolidine system. Reaction volume = 3.4 mL. Light path = 1 cm.
Warnings
Chromatographically purified
Synonyms
EC 1.1.3.9; D-galactose oxidase; β-galactose oxidase; 9028-79-9; Galactose Oxidase