Description
Bromoperoxidase from Corallina officinalis is a phenoxazine dye. The brilliant cresyl blue (BCB) test determines the activity of glucose-6-phosphate dehydrogenase (G6PDH). The activity of this enzyme is greatest in growing oocytes and declines as oocytes mature. It stains reticulocytes and trichomonads. Bromoperoxidase contains a significant amount of nonheme iron. It is activated by vanadate ions. Maximal activity is achieved with stoichiometric vanadium incorporation.
Abbr
BCB, Native (Corallina officinalis)
Source
Corallina officinalis
Applications
Bromoperoxidase from Corallina officinalis may be used for staining brain tissue, nuclei, plant chromosomes, reticulocytes, platelets and reticulated red cells. It may be used for the detection of biochemical molecules and the BCB enzyme assay. The BCB assay is also used industrially in optical data storage.
Form
Partially purified, lyophilized powder containing MES buffer salts
Activity
> 100 units/mg protein (Lowry)
Unit Definition
One unit will catalyze the conversion of 1.0 μmole of monochlorodimedon to monobromochlorodimedon per min at pH 6.4 at 25°C.
Synonyms
BCB; Bromide Peroxidase; Bromoperoxidase; 69279-19-2
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