Description
Choloylglycine hydrolase (EC 3.5.1.24) is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, libeRates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg2+, Cu2+, and Cd2+) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.
Abbr
Choloylglycine Hydrolase, Native (Clostridium perfringens (C. welchii))
Source
Clostridium perfringens (C. welchii)
Applications
The enzyme from Creative Enzymes has been used in the analysis of bile samples in various studies.
Form
lyophilized powder. Partially purified lyophilized powder containing buffer salts and stabilizer
Enzyme Commission Number
EC 3.5.1.24
Activity
> 100 units/mg protein
Unit Definition
One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37°C.
Synonyms
EC 3.5.1.24; glycocholase; bile salt hydrolase; choloyltaurine hydrolase; 3α,7α,12α-trihydroxy-5β-cholan-24-oylglycine amidohydrolase; 37289-07-9
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