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Native Bovine Malic Dehydrogenase
Cat No.
NATE-0445
Description
Malic dehydrogenase (MDH) exists as two isoforms within eukaryotic cells, one that is expressed in the mitochondria and functions in the TCA cycle and one in the cytoplasm that converts malate from the mitochondria back into oxaloacetate.
Abbr
MDH, Native (Bovine)
Alias
malic enzyme
Source
Bovine heart
Species
Bovine
Applications
Malic dehydrogenase has been used in a study to assess a flow injection system for on-line monitoring of fumaric acid in biological pr ocesses. 1 It has also been used in a study to investigate a root-knot nematode parasitizing peanut in Texas.
Product Overview
Malic Dehydrogenase from bovine heart contains a histidine residue at the NAD-binding active site which is critical for activity. When this histidine is mutated a loss in activity is observed.
Form
ammonium sulfate suspension; Suspension in 3 M (NH4)2SO4-0.01 M KH2PO4 solution, pH 7.3
Enzyme Commission Number
EC 1.1.1.37
Activity
2000-4000 units/mg protein (modified Warburg-Christian)
CAS No.
9001-64-3
Contaminants
<0.01% Glutamic-Oxalacetic Transaminase; <0.01% Glutamic-Pyruvic Transaminase
Unit Definition
One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25°C.
Storage
2-8°C
Synonyms
malic dehydrogenase; L-malate dehydrogenase; NAD-L-malate dehydrogenase; malic acid dehydrogenase; NAD-dependent malic dehydrogenase; NAD-malate dehydrogenase; NAD-malic dehydrogenase; malate (NAD) dehydrogenase; NAD-dependent malate dehydrogenase; NAD-specific malate dehydrogenase; NAD-linked malate dehydrogenase; MDH; L-malate-NAD+ oxidoreductase; EC 1.1.1.37; 9001-64-3
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1107 | L-Malate dehydrogenase from E. coli, Recombinant | EC 1.1.1.37 | 9001-64-3 | E. coli | Inquiry |
| NATE-0448 | Native Thermus flavus Malic Dehydrogenase | EC 1.1.1.37 | 9001-64-3 | Thermus flavus | Inquiry |
| DIA-278 | Native Porcine Malate Dehydrogenase, IFCC Quality | Porcine heart | Inquiry | ||
| NATE-1030 | Native Yeast Malate Dehydrogenase | EC 1.1.1.37 | 9001-64-3 | Yeast | Inquiry |