Description
Lactoperoxidase catalyzes the oxidation of iodide to iodine by hydrogen peroxide. This activity provides a gentle, specific alternative to chloramine T for the radioiodination of proteins and DNA.
Abbr
LPO, Native (Bovine)
Form
Type I, ammonium sulfate suspension, Suspension in 3.2 M (NH4)2SO4 pH approx. 7.0; Type II, Type IV, lyophilized powder; Type III, lyophilized powder (essentially salt-free).
Enzyme Commission Number
EC 1.11.1.7
Activity
Type I, > 50 units/mg protein; Type II, > 150 units/mg; Type III, > 200 units/mg protein; Type IV, > 80 units/mg protein.
Unit Definition
One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20°C.
Warnings
Using 2,2′-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt as substrate, two or three times the activity is observed.
Pathway
Salivary secretion, organism-specific biosystem; Salivary secretion, conserved biosystem
Function
calcium ion binding; heme binding; thiocyanate peroxidase activity
Synonyms
lactoperoxidase; guaiacol peroxidase; plant peroxidase; Japanese radish peroxidase; horseradish peroxidase (HRP); soybean peroxidase (SBP); extensin peroxidase; heme peroxidase; oxyperoxidase; protoheme peroxidase; pyrocatechol peroxidase; scopoletin peroxidase; Coprinus cinereus peroxidase; Arthromyces ramosus peroxidase; EC 1.11.1.7; 9003-99-0; LPO; SPO
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