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Native Baker's yeast (S. cerevisiae) Phosphoglucose Isomerase

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Cat No.

NATE-0554

Description

Phosphoglucose Isomerase (PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis. It is highly conserved in bacteria and eukaryotes.

Abbr

PGI, Native (Baker's Yeast (S. cerevisiae))

Alias

PGI

Source

Baker's yeast (S. cerevisiae)

Applications

Isomerization of ketoses to aldoses

Enzyme Commission Number

EC 5.3.1.9

Activity

350 U/mg at +25°C with F6P as substrate.

CAS No.

9001-41-6

Contaminants

< 0.01% F6P-K, GR, 6-PGDH and PGIuM each < 0.2% B-fructosidase.

Molecular Weight

145 kDa

Unit Definition

One unit (U) phosphoglucose isomerase will produce 1 mol of glucose-6-phosphate from fructose-6-phosphate in 1 min at +25°C and pH 7.6 (triethanolamine buffer). The above assay produces 1umol of NAD (P)H per umol of glucose-6-phosphate formed.

Optimum pH

7.0-7.6

Storage

Stable at +2 to +8°C.

Buffer

Suspension in 3.2 M ammonium sulfate solution, pH approx. 6

Inhibitors

Pyridoxal-5’-phosphate

Synonyms

Glucose-6-phosphate isomerase; EC 5.3.1.9; phosphohexose isomerase; phosphohexomutase; oxoisomerase; hexosephosphate isomerase; phosphosaccharomutase; phosphoglucoisomerase; phosphohexoisomerase; phosphoglucose isomerase; glucose phosphate isomerase; hexose phosphate isomerase; D-glucose-6-phosphate ketol-isomerase; 9001-41-6; PGI

"PGI" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1119	Phosphoglucose isomerase from Saccharomyces cerevisiae, Recombinant	EC 5.3.1.9	9001-41-6	Saccharomyces cerevisiae	Inquiry
NATE-1117	Phosphoglucose isomerase from Bacillus subtilis, Recombinant	EC 5.3.1.9	9001-41-6	Bacillus subtilis	Inquiry
NATE-1118	Phosphoglucose isomerase from E.coli, Recombinant	EC 5.3.1.9	9001-41-6	E.coli	Inquiry
DIA-162	Native Bacillus stearothermophilus Phosphoglucose isomerase	EC 5.3.1.9	9001-41-6	Bacillus stearothermophilus	Inquiry

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