Description
A thermostable 4-α-glucanotransferase that elongates linear alpha-glucan chains in starch and amylose by catalyzing the transfer of one glucose unit from the non-reducing end to a new position in an acceptor, which may be triose or larger. The enzyme can be used various applications, such as for modifications of alpha-polysaccharides and is ideal for making clear size ladders of oligosaccharides larger than maltose. The enzyme was developed from metagenome DNA obtained from environmental sample from a geothermal environment.
Abbr
Glucan elongation enzyme, Native (Bacterial Environmental DNA)
Source
Bacterial Environmental DNA
Product Overview
The enzyme catalyzes the formation of the alpha-1,4-glucosidic linkages. The enzyme transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.
Unit Definition
Half unit (U) is defined as the amount of enzyme which lowers the OD at 650 nm from 1.2 to 0.7 in 30minutes.
Optimum pH
pH optimum is around 7
Optimum temperature
The enzyme in relatively active in a rather broad temperature range with optimum around 70°C
Synonyms
Glucan elongation enzyme
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