Description
Lysyl endopeptidase (EC 3.4.21.50, Achromobacter proteinase I, Achromobacter lyticus alkaline proteinase I, protease I, achromopeptidase, lysyl bond specific proteinase) is an enzyme. This enzyme catalyses the following chemical reaction:Preferential cleavage:Lys-, including-Lys-Pro-. This enzyme is isolated from Achromobacter lyticus.
Abbr
Achromopeptidase, Native (Bacteria)
Applications
α-Hemolysin was used in a study to test the efflux pump and haemolysin activity of Escherichia coli of dairy origin. It was also used to test its adaptation to benzalkonium chloride and the effect of ciprofloxacin on biofilm formation.
Product Overview
Hemolysins are lipids and proteins that cause lysis of red blood cells by destroying their cell membrane. Although the lytic activity of some microbe-derived hemolysins on red blood cells may be of great importance for nutrient acquisition, many hemolysins produced by pathogens do not cause significant destruction of red blood cells during infection. Although hemolysins are capable of doing this for red blood cells in vitro.
Enzyme Commission Number
EC 3.4.21.50
Activity
> 1,000 units/mg solid; > 20,000 units/mg solid
Unit Definition
One unit will produce a change in A600 of 0.001 per minute per mL at pH 8.0 at 37°C using a suspension of Micrococcus lysodeikticus as substrate (1 cm light path).
Buffer
H2O: soluble 0.49-0.51 mg/mL
Synonyms
Achromobacter proteinase I; Achromobacter lyticus alkaline proteinase I; protease I; achromopeptidase; lysyl bond specific proteinase; EC 3.4.21.50; 123175-82-6; Lysyl endopeptidase
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