Description
In molecular biology, acetate kinase (EC 2.7.2.1), which is predominantly found in micro-organisms, facilitates the production of acetyl-CoA by phosphorylating acetate in the presence of ATP and a divalent cation. Short-chain fatty acids (SCFAs) play a major role in carbon cycle and can be utilized as a source of carbon and energy by bacteria. The enzyme is important in the process of glycolysis, enzyme levels being increased in the presence of excess glucose. The growth of a bacterial mutant lacking acetate kinase has been shown to be inhibited by glucose, suggesting that the enzyme is involved in excretion of excess carbohydRate. A related enzyme, butyRate kinase, facilitates the formation of butyryl-CoA by phosphorylating butyRate in the presence of ATP to form butyryl phosphate
Abbr
Acetate kinase, Native (Bacillus stearothermophilus)
Source
Bacillus stearothermophilus
Applications
Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Bacillus stearothermophilus has been used to study allosteric activation,[32P]-acetyl phosphate was generated by incubating potassium acetate in the reaction mixture with acetate kinase from Creative Enzymes. This [32P]-acetyl phosphate was used to label BldM, BldM D-54N or BldM D-54A loci during the study of the effect of bldM gene on Streptomyces coelicolor development.
Form
lyophilized powder. Contains potassium phosphate buffer
Enzyme Commission Number
EC 2.7.2.1
Activity
400-1,200 units/mg solid
Unit Definition
One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.2 at 30°C.
Synonyms
Acetate kinase (phosphorylating); Acetic kinase; Acetokinase; EC 2.7.2.1; 9027-42-3; Acetate kinase
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