Description
In enzymology, a choline oxidase (EC 1.1.3.17) is an enzyme that catalyzes the chemical reaction: choline + O2↔ betaine aldehyde + H2O2. Thus, the two substrates of this enzyme are choline and O2, whereas its two products are betaine aldehyde and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor.
Abbr
Choline Oxidase (Alcaligenes sp.)
Applications
This enzyme is useful for enzymatic determination of phospholipids when coupled with phospholipase D and for choline esterase-activity in clinical analysis.
Appearance
Yellowish amorphous powder, lyophilized
Enzyme Commission Number
EC 1.1.3.17
Activity
GradeIII 10U/mg-solid or more (containing approx. 20% of stabilizers)
Contaminants
Catalase < 1.0×10²%
Molecular Weight
approx. 95 kDa
Isoelectric point
4.1±0.1
pH Stability
pH 7.0-9.0 (30°C, 2 hr)
Michaelis Constant
2.84×10⁻³M (Choline), 5.33×10⁻³M(Betaine aldehyde)
Structure
One mol of FAD is covalently bound to mol of the enzyme
Optimum temperature
40-45°C
Thermal stability
below 37°C (pH 7.5, 10min)
Stability
Stable at-20°C for at least 6 months
Stabilizers
EDTA, bovine serum albumin, amino acids (glycine, sodium gluta-mate, etc.)
Inhibitors
p-Chloromercuribenzoate, Cu⁺⁺, Co⁺⁺, Hg⁺⁺, Ag⁺
Synonyms
choline oxidase; EC 1.1.3.17
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