Description
NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis.
Abbr
NADK (catalytic domain), Recombinant (Human)
Enzyme Commission Number
EC 2.7.1.23
Molecular Weight
~42 kDa (monomer). Human NAD kinase (aa 64-446) is fused at the N-terminus to a His-tag.
Unit Definition
One unit is defined as the amount of enzyme that synthesizes 1 µmol of NADP per min.
Storage
Prepare aliquots and store at -20°C. Avoid repeated freeze/thaw cycles.
Synonyms
NADK; Poly (P)/ATP NAD Kinase; EC 2.7.1.23
Reconstitution
Prepare working aliquots by centrifugation and dissolve the pellet in 50 mM Tris-HCl pH 7.5, 150 mM sodium chloride and 1 mM DTT.
Formulation
2 mg/mL solution in 20 mM Tris, pH 8 containing 20% glycerol.
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