Product Overview
N3PT is a potent and selective TK inhibitor both in vitro and in vivo.
Synonyms
N3-pyridyl thiamine
IC50
22 nM(Apo-TK); 26 nM (EC50, Cellular TK)
Molecular Formula
C13H19Cl2N3OS
Chemical Name
2-[3-[(2-amino-6-methylpyridin-3-yl)methyl]-4-methyl-1,3-thiazol-3-ium-5-yl]ethanol; chloride; hydrochloride
Solubility
Soluble in DMSO
Shipping Conditions
Evaluation sample solution: ship with blue ice. All other available size: ship with RT, or blue ice upon request
In vitro
N3PT inhibits transketolase activity in a cell based assay. Competitive inhibition of TK by N3PT in cells treated with increasing doses of thiamine, expressed as percentage enzymatic activity (the slope of initial linear range) of controls not treated with compounds.
In vivo
Tumors were induced in mice at day 0 and mice were then treated at day 7 with vehicle alone or with N3PT. Low-thiamine diet enhances the sensitivity to N3PT inhibition of TK in spleen. Animals were switched to diets containing 16.5 mg/kg (unchanged), 5 mg/kg, 1 mg/kg, or 0 mg/kg thiamine, from a normal chow containing 16.5mg/kg thiamine.
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