Description
Mutant Alanine Racemase (Y354N) (mAR-Y354N), a pyridoxal 5-phosphate (PLP) dependent enzyme catalyzes the interconversion of the L-Serine to D-Serine. In WT Alanine Racemase Tyr354 plays a crucial role in defining the strict specificity of AR for alanine, in converting L-Alanine to D-Alanine, which is an important component of the peptidoglycan layer of bacterial cell wall. By mutating the active site Tyr 354 to Asn, the specificity of the enzyme changes and it becomes a racemase with dual specificity for L- Alanine and L-Serine.
Abbr
mAR-Y354N, Recombinant (G. stearothermophilus)
Source
E. coli and fused to His-tag at N-terminus
Species
Geobacillus stearothermophilus
Applications
Mutant Alanine racemase Y354N can be used to convert L-serine to D-serine and L-Alanine to D-Alanine.
Enzyme Commission Number
EC 5.1.1.1
Unit Definition
One unit enzyme converts 1 µmole of L-serine into D-serine per minute at 37°C and pH 7.4 in the presence of pyridoxal phosphate.
Storage
Reconstituted enzyme can be stored in working aliquots at -20°C and use within 3 months. Avoid repeated freeze-thaw cycles.
Synonyms
Alanine Racemase Y354N; alr; dal
Reconstitution
Reconstitute enzyme in 50 mM phosphate buffer, pH 7.4.
Formulation
1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl and 10% glycerol.
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