Description
λ Protein Phosphatase (λ-PPase) is a Mn+2-dependent protein phosphatase with activity towards phosphorylated serine, threonine, tyrosine and histidine residues. It is the 221 amino-acid product of ORF221 open reading frame on bacteriophage lambda (1, 2). λ-PPase was expressed as a recombinant protein in E.coli and highly purified (2). This product is an intact enzyme of high quality without tag.
Abbr
λ-PPase, Recombinant
Applications
λ-PPase can be used to release phosphate groups from phosphorylated serine, threonine, tyrosine and histidine residues in proteins (2). It should be noted that different proteins are dephosphorylated at different rates. Optimal reaction temperature is 30°C. Inclusion of protease inhibitor cocktail and shortest incubation time is desired when assays are done with crude samples.
Form
400 U/ul λ-PPase in 50mM HEPES (pH 7.5), 100mM NaCl, 2mM dithiothreitol, 0.1 mM MnCl2, 0.1mM EDTA, 50% glycerol, 0.01% Brij 35.
Activity
400,000 U/mg (400 U/ul)
Unit Definition
One unit is defined as the amount of enzyme that hydrolyzes 1 nmole of p-nitrophenyl phosphate per minute at 30°C.
Storage
Store at -80°C. Avoid repeating freeze-thaw cycles.
Synonyms
Protein phosphatase 1F; PPM1F; CAMKP; CaMKPase; FEM-2; POPX2; hFEM-2; Lambda Protein Phosphatase; λ-PPase
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