Description
Lacto-N-Biosidase may be used during glycoprotein analysis to better study the structure and function of glycoprotein and glycolipid sugar chains. This product can be used for specific oligosaccharide hydrolysis of type-I chain oligosaccharides, producing lacto-N-biose (Gal β 1-3 GlcNAc) as a byproduct, but not oligosaccharide hydrolysis of type-II chain oligosaccharides. As a result, Lacto-N-Biosidase can be used to distinguish type-I versus type-II glycoprotein as well as glycolipid sugar chains. When used in conjunction with α -1,3/4-Fucosidase, Lacto-N-Biosidase can also help distinguish Sialyl-Lewis x and Sialyl-Lewis a structures.
Abbr
Lacto-N-Biosidase, Recombinant
Source
Streptomyces sp. 142
Applications
Specific hydrolysis of oligosaccharides with type-I sugar chains and production of byproduct lacto-N-biose (Gal β 1-3 GlcNAc)
Form
Solution in 50 mM sodium acetate buffer, pH 5.5, containing 0.05% Brij-58
Molecular Weight
60 kDa (SDS-PAGE)
pH Stability
pH 4.0–10 (4°C, 16 hrs.)
Unit Definition
One unit is the amount of enzyme required to hydrolyze 1 µmol of PA-lacto-N-tetraose in 1 minute at 37°C, pH 5.5.
Synonyms
Oligosaccharide lacto-N-biosylhydrolase; Lacto-N-Biosidase
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