Description
KOD is a high fidelity thermostable DNA polymerase that amplifies target DNA up to 6 kbp with superior accuracy and yield for PCR applications. The enzyme's 3'→5' exonuclease-dependent proofreading activity results in a lower PCR mutation frequency than any other commercially available DNA polymerase. The elongation rate and processivity are 5 times and 10 to 15 times higher, respectively, than for Pfu DNA polymerase, resulting in highly accurate and robust yield, in a short reaction time.
Abbr
KOD DNA Polymerase, Recombinant (Thermococcus kodakaraensis)
Species
Thermococcus kodakaraensis
Product Overview
Higher fidelity than Pfu DNA polymerase—excellent for cloning
Greater yield—extension speed is 2X faster than Taq DNA polymerase and 5X faster than Pfu DNA polymerase
Higher processivity—sequential nucleotide polymerization is 10- to 15-fold greater than Pfu and Tli DNA polymerases
Amplifies plasmid and lambda DNA templates up to 6 kbp
Amplifies genomic DNA templates up to 2 kbp
No truncated amplification products
Purity
> 90% homogeneous by SDS-PAGE
Unit Definition
One unit of enzyme is defined as the amount of enzyme that will incorporate 10 nmoles of dNTPs into acid insoluble material in 30 minutes at 75°C.
Formulation
50 mM Tris, pH 8.0, containing 500 mM sodium chloride, 5% glycerol, and 1 mM DTT
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