Description
SlyD is a putative folding helper protein from the Escherichia coli cytosol, which has N-terminal prolyl isomerase domain of the FKBP type and a most likely unstructured C-terminal tail. SlyD is an important factor in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase.
Appearance
Sterile Filtered colorless solution.
Product Overview
SlyD Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 196 amino acids and having a molecular mass of 21 kDa.
Activity
> 66 nmoles/min/ug
Purity
Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Unit Definition
Specific activity is defined as the amount of enzyme that cleave 1umole of suc-AAFP-pNA per minute at 1C in Tris-Hcl pH8.0 using chymotrypsin.
Stability
SlyD although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Buffer
SlyD protein solution contains 20mM Tris pH-7.5.
Synonyms
FKBP-Type Peptidyl-Prolyl Cis-Trans Isomerase; SlyD
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