Description
Insulin Degrading Enzyme (IDE) is a large zinc-binding protease of the M16A metalloprotease subfamily known to cleave multiple short polypeptides that vary considerably in sequence. IDE was first identified by its ability to degrade the B chain of the hormone insulin. This activity was observed over sixty years ago, though the enzyme specifically responsible for B chain cleavage was identified more recently. This discovery revealed considerable amino acid sequence similarity between IDE and the previously characterized bacterial protease pitrilysin, suggesting a common proteolytic mechanism.
Abbr
IDE, Recombinant (Rat)
Product Overview
Recombinant, rat insulin degrading enzyme fused to a His•Tag sequence and expressed in S. frugiperda insect cells. A metalloprotease that degrades insulin and a variety of other peptides including amyloid peptides.
Enzyme Commission Number
EC 3.4.24.56
Unit Definition
One unit is defined as the amount of the enzyme that will catalyze the hydrolysis of 1 µmol iodinated insulin per h at 37°C, pH 7.3.
Buffer
In 100 mM potassium phosphate buffer, 20% glycerol, pH 7.3.
Synonyms
IDE; Insulin-degrading enzyme; insulysin; insulin protease
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