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Hyaluronate lyase from Streptomyces coelicolor, Recombinant

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Cat No.

NATE-1211

Description

Hyaluronate lyase degrades hyaluronate using an elimination reaction to break glycosidic linkages yielding unsaturated disaccharide products.

Abbr

Hyaluronate Lyase, Recombinant (Streptomyces coelicolor)

Source

Streptomyces coelicolor A3(2)

Form

Supplied in 3.2 M ammonium sulphate

Enzyme Commission Number

EC 4.2.2.1

Activity

8.1 U/mg

CAS No.

37259-53-3

Molecular Weight

82914.2 Da

Purity

> 95 % as judged by SDS-PAGE

Concentration

4.8 U/ml

Unit Definition

One unit is defined as the amount of enzyme required to release 1μmol of 4,5-unsaturated product per minute from 1 mg/mL hyaluronic acid in 50 mM sodium acetate buffer, pH 6.0, at 37°C, as measured at 232 nm.

Optimum pH

~ 6.0

Optimum temperature

37°C

Storage

Store at 4°C (shipped at room temperature)

Preparation Instructions

Agitate vial sufficiently to fully homogenise enzyme precipitate before use.

Synonyms

hyaluronidase; glucuronoglycosaminoglycan lyase; spreading factor; mucinase; 37259-53-3; EC 4.2.2.1; hyaluronate lyase

"Hyaluronate Lyase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0346	Hyaluronate Lyase from Streptococcus pyogenes, Recombinant	EC 4.2.2.1	37259-53-3	E. coli	Inquiry
NATE-1210	Hyaluronate lyase from Streptococcus equi, Recombinant	EC 4.2.2.1	37259-53-3	Streptococcus equi 4047	Inquiry
EXWM-5084	hyaluronate lyase	EC 4.2.2.1	37259-53-3		Inquiry